AMI plasma EV-miRNA-mRNA targets (IPA, Qiagen) are significantly over represented when compared to neutrophil Gene Ontology terms for degranulation ( P 0.65 and median difference >1, and 47 miRNAs with AUC >0.65 and mean difference >1. Human plasma EV-miRNAs are significantly altered post-AMI. Experimental AMI in wild type, naïve (C57B6/J) mice induces splenic-neutrophil deployment ( P = 0.004). Plasma EVs at the time of presentation correlate significantly with the extent of ischemic injury ( R = 0.046, P = 0.006) and plasma neutrophils ( R = 0.37, P = 0.017). a 6-month follow-up measurement ( P = 0.008). Results: Patients presenting with AMI ( n = 15) have 2.2-fold more plasma EV at time of injury vs. Mouse and human EC were used in vitro to derive EC-EV. EV were isolated using ultra centrifugation (120,000 g 2 h) and characterized for size and concentration by Nanoparticle Tracking Analysis, EV markers (TSG101, ALIX, CD63/CD69) by western blot, and microRNAs (miRNAs) by RT-qPCR. Methods: Patients provided informed consent as part of the Oxford Acute Myocardial Infarction Study. Here, we describe neutrophil deployment from the spleen in AMI and by endothelial cell (EC)-derived EVs. Neutrophils also reside in the spleen and are the first cells to arrive at sites of injury and mediate further damage.
Introduction: Acute myocardial infarction (AMI) mobilizes monocytes from the splenic reserve and induces transcriptional activation en route to the injured myocardium, possibly through interactions involving plasma liberated extracellular vesicles (EVs).
Brian Byrd Pia SiljanderLocation: Level B1, Hall B 11:00–12:30Įxtracellular vesicles mediate neutrophil cell deployment from the spleen following acute myocardial infarction Symposium Session 1: Cardiovascular Disease Thursday 25 April 2019 Chairs: J. Based on the concept of emergent properties of heterogeneous extracellular vesicles, future research directions to decode the complexity of extracellular vesicle-mediated intercellular communication network, either at the single vesicle level or at a systems level as a whole, and the secret of life will be briefly introduced. Furthermore, bacterial extracellular vesicle-based cancer immunotherapy will be introduced. Then, this presentation focuses on our recent progress in novel extracellular vesicle-mimetic technologies for targeted drug delivery, theranostics and epigenetic reprogramming as well as for adjuvant-free, non-toxic vaccine delivery system against bacterial infection. This presentation briefly introduces our last 20 year’s comprehensive research on extracellular vesicles derived from host, bacteria, diet and environments including their physical, biochemical and biological complex properties (). Recent progress in this area has revealed that extracellular vesicles play multifaceted pathophysiological functions by delivering the complex messages between cells and organisms, suggesting that extracellular vesicles are NanoCosmos, i.e., extracellular organelles that play diverse roles in intercellular and interkingdom communication. The secretion of nanosized lipid bilayered extracellular vesicles is a universal cellular process occurring from simple organisms to complex multicellular organisms. NanoCosmos: extracellular vesicles as nanosized extracellular organelles delivering the complex messages between cells and organismsĭepartment of Life Sciences, POSTECH, Pohang, Republic of Korea Plenary Session 1: Standardizations Chairs: Andrew Hill Hidetoshi TaharaLocation: Level 3, Hall B
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